No Items In Cart
Dimethyl sulfoxide (DMSO) is a common ingredient in cryopreservation media. In the literature, DMSO has been noted to be incompatible with polycarbonate as bisphenol A (BPA) (an endocrine disrupter) is a possible extractable – a toxicological concern even in low concentrations. Understanding this, West undertook examining the interaction of cell media and different percentage DMSO solutions with VAs.
West used microscopy and gas chromatography–mass spectrometry (GC/MS) extractable techniques to analyze the effects of DMSO in concentrations typically used in cryopreservation media (≤ 15%). As expected, VAs showed dramatic visual effects via microscopy with as little as one minute of exposure to 100% DMSO, but no visual effects were observed in VAs exposed to 10% DMSO, 15% DMSO, or Cryostor® CS10 media out to 60 minutes. Furthermore, extractables studies suggest VA exposure to low percentage concentrations of DMSO (10% DMSO and simulated 15% DMSO) up to 60 minutes do not result in BPA as an extractable.
Vented (VVAs) and non-vented VAs were also tested for deliverable and residual volume performance in comparison to a 27 gauge needle. VVAs and VAs performed comparably for both low (1 cP) and high (15 cP) viscosity solutions. Furthermore, West compared the performance of the VVA versus a 27 gauge needle for the retrieval of suspension CD34+ cells (HSCs) and adherent human mesenchymal stem cells (hMSCs) from 2mL Daikyo Crystal Zenith® vials. West’s VVAs were capable of volume and cell recovery comparable to that of a 27 gauge needle and syringe across four different operators and both cell types.
These results are described in a recent Technical Report, Evaluation of West’s Vial Adapters for Cell Therapy Applications, Technical Report 2020/224. West’s VAs are compatible with DMSO-containing cell therapy medias and perform as well as needles for withdrawal of cell solutions as tested. For more, visit the Knowledge Center or contact a Technical Customer Support (TCS) representative.
Crystal Zenith® is a registered trademark of Daikyo Seiko, Ltd. Daikyo Crystal Zenith technology is licensed from Daikyo Seiko, Ltd.
Cryostor® is a registered trademark of BioLife Solutions, Inc.